Composite

Part:BBa_K4011017:Design

Designed by: Zhuhan Liu   Group: iGEM21_LINKS_China   (2021-10-21)


pRPL8B-AeAT9-tSSA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
    Illegal BglII site found at 912
    Illegal BglII site found at 1530
    Illegal XhoI site found at 2029
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2015
    Illegal BsaI.rc site found at 2265
    Illegal SapI.rc site found at 1777


Design Notes

1. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias.

2. BsaI restriction sites were added to the three basic parts used to construct pRPL8B-AeAT9-tSSA1 for Golden Gate assembly.

3. BsmB1 sites flank the composite part for further Golden Gate assembly construction.


Source

pRPL8B and tSSA1 all come from S. cerevisiae, while AeAT9 comes from Actinidia eriantha.

References